Fig. 1: Selective targeting of VTAglutamate-GABA, VTAglutamate-only and VTAGABA-only neurons. | Nature Communications

Fig. 1: Selective targeting of VTAglutamate-GABA, VTAglutamate-only and VTAGABA-only neurons.

From: VTA monosynaptic connections by local glutamate and GABA neurons and their distinct roles in behavior

Fig. 1

A Crossing of vglut2-Cre mice with vgat-Flp mice to generate vglut2-Cre/vgat-Flp mice (reproduced from ref. 5). B–D Intra-VTA injections of INTRSECT AAV-Con/Fon-ChR2-eYFP to target VTAglutamate-GABA neurons (B), AAV-Con/Foff-ChR2-eYFP to target VTAglutamate-only neurons (C), or AAV-Coff/Fon-ChR2-eYFP vectors to target VTAGABA-only neurons (D). E VTAglutamate-GABA neurons co-expressing eYFP, VGluT2 and VGaT mRNAs, VTAglutamate-only neurons expressing eYFP and VGluT2 mRNA without VGaT mRNA and VTAGABA-only neurons expressing eYFP and VGaT mRNA without VGluT2 mRNA. F Detection of VGluT2 and VGaT mRNAs within the subpopulation of VTA neurons co-expressing eYFP. G Detection of VGluT2 mRNAs within the subpopulation of VTA neurons co-expressing eYFP. H Detection of VGaT mRNAs within the subpopulation of VTA neurons co-expressing eYFP. Data are shown as mean ± SEM. The number of total counted neurons (“n”) is shown in each pie graph (three mice/group). Source data are provided as a Source Data file.

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