Fig. 5: NE temporal patterning extends to other OPC spatial compartments and temporal windows.
From: Concurrent temporal patterning of neural stem cells in the fly visual system
a–d Imp (green, a and c) and Syp (red, b and d) expression in OPC NBs (Dpn, gray) of the early and late third instar larval brain. e–h Exd (red), Ey (green), and Slp2 (blue) in OPC NBs at 72 h ALH (e), 96 h ALH (f), 0 h APF (g), and 5 h APF (h). Gray dashed line marks the boundary between the OPC NE and NBs. White arrow illustrates the progression of the NB tTF series. i and j Expression of Exd (red, i), Ey (green, j) and Slp2 (blue, j) in OPC NBs (Dpn, gray) of an imp LOF mutant. k and l Expression of Exd (red, k), Ey (green, l) and Slp2 (blue, l) in sypRNAi LOF NBs. sypRNAi is driven in cOPC NBs by MzVUM-Gal4. The cOPC (GFP, gray) boundary is marked by white dashed lines. m–v The developmental birth origins of Dm4 (m–q) and Tm26 (r–v) neurons. Schematics illustrating the spatial–temporal birth origin (m and r), morphology (o and t), and cell body positions (p and u) of Dm4 and Tm26 neurons are on the left. Dm4 and Tm26 neurons are labeled in the adult optic lobe (Brp, magenta) by Dm4-Gal4 > GFP (green, n) and Tm26-Gal4 > GFP (green, s), respectively. White arrowheads denote neuronal cell bodies and white dashed lines mark medulla layer M7. 60–92 h ALH EdU feeds (red, q and v) label Dm4 (white dashed circles, q”) but not Tm26 (white dashed circles, v”) neurons. Region of the medulla cortex outlined with white dashed lines (q and v) is magnified in the images below. w The proportion of Dm4 neurons (green) and Tm26 neurons (red) labeled by EdU administered from 60–92 h ALH (two-sided two-proportion Z-test, P < 0.00001, n = 409 Dm4 neurons and 164 Tm26 neurons). Error bars denote SEP. Source data are provided as a Source Data file. x Schematic depicting the developmental birth windows of Dm4 and Tm26 neurons. In all images and schematics: Anterior/medial is left. Dorsal is up in (a–l, m, p, r, u and x). Scale bar: 15 µm.
