Fig. 6: Uni-columnar neurons are specified independently of NE temporal patterning.
From: Concurrent temporal patterning of neural stem cells in the fly visual system
a Schematic illustrating the spatial–temporal birth origin of Mi1 (green) and Tm3 (orange) uni-columnar neurons in the larval OPC. b Schematic depictions of Mi1 and Tm3 neurons in the optic lobe. c and d Schematics displaying Mi1 (c) and Tm3 (d) cell body positions in the medulla cortex. e 60–92 h ALH EdU feeds (red) label Mi1 neurons (Bsh, green) in the anterior (e’–e”), but not posterior (e”’–e””) medulla cortex. Cell bodies are outlined with white dashed circles. f 70–78 h ALH EdU feeds (red) label Tm3 neurons (Tm3-Gal4 > GFP, green) in the anterior (f’–f”), but not posterior (f”’–f””) medulla cortex. Cell bodies are outlined with white dashed circles. g–j Mi1 neurons (Bsh, green) in the developing optic lobe at different stages of neurogenesis. Arm (magenta) labels the OPC NE, and white arrows indicate the direction of proneural wave propagation. The youngest Mi1 neurons at each stage are denoted with a yellow arrowhead. Lamina neurons are also labeled by Bsh (white arrow, i and j). k and l Mi1 neurons (Bsh, green) in a wild-type (k) and imp LOF (l) optic lobe at 5 h APF. DE-Cad (gray) labels the OPC NE. m Mi1 neurons (Bsh, green) in sypRNAi LOF clones (RFP, red) at 5 h APF. Yellow dashed lines mark the boundary of the clone. n Schematic depicting the developmental birth windows of Mi1 and Tm3 neurons. In all images: Medial/anterior is left. Dorsal is up in (a, c, d and n). Scale bar: 15 µm.
