Fig. 2: RNAscope validation of candidate mRNA gene expression for each astrocyte subtype.
From: Aging in mice alters regionally enriched striatal astrocytes
Representative images (left) of mouse central striatum with mRNA for Atp1a2 (a), Crym (b), Cplx2 (c), Aqp4 (d), Pdgfa (e), Rgs4 (f), and Gfap (g) in red, and S100β astrocyte marker protein in green. Quantification of the mRNA puncta intensity and the number of mRNA puncta per cell for each gene of interest in the dorsal, central, and ventral striatum (right). Average data shown as mean ± SEM. from n  =  30–35 cells from 3 mice (one-tailed one-way ANOVA followed by Tukey’s post hoc test or Kruskal-Wallis ANOVA). Source data are provided. In the case of Pdgfa, Rgs4, and Cplx2, mRNA was also detected in cells that were not S100β-positive astrocytes. These were NeuN-positive neurons. This is consistent with the idea that individual genes are not categorical markers but are part of a set of genes used to define cell clusters.
