Table 2 MS/MS analyses of HUWE1HECT-driven compound ubiquitination

From: Selective ubiquitination of drug-like small molecules by the ubiquitin ligase HUWE1

compound

-

-

BI8622

BI8626

1

ATP

-

+

+

+

+

ESTLHLVLRLRGG-BI8622

X

X

yes

X

X

QLEDGRTLSDYNIQKESTLHLVLRLRGG-BI8622

X

X

yes

X

X

ESTLHLVLRLRGG-BI8626

X

X

X

yes

X

QLEDGRTLSDYNIQKESTLHLVLRLRGG-BI8626

X

X

X

yes

X

ESTLHLVLRLRGG-1

X

X

X

X

yes

QLEDGRTLSDYNIQKESTLHLVLRLRGG-1

X

X

X

X

yes

  1. Table summarizing LC-MS/MS analyses of HUWE1HECT-driven multi-turnover ubiquitination reactions, containing the indicated compounds. The identification of the specified C-terminal Ub-derived peptides, resulting from LysC protease digestions with no or one missed cleavage site, respectively, is indicated by 'yes'. For the corresponding, annotated MS/MS spectra, see Supplementary Figs. 6–8.
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