Fig. 3: Supplemental activation with electroporation+roscovitine can rescue the fertilization failure.
From: Induction of experimental cell division to generate cells with reduced chromosome ploidy
a Schematic of the MII arrest mechanism and rescue of SCNT oocyte fertilization by supplemental activation with electroporation+roscovitine. Created in BioRender. Marti, N. (2025). b Activation and embryo development in control and SCNT oocytes after activation. Elec.- electroporation. Rosc-roscovitine treatment. Bar plots show the mean of 4 (MII with electroporation alone group), 5 (SCNT with electroporation alone group), 6 (MII with electroporation+roscovitine group) and 7 (SCNT with electroporation+roscovitine group) independent replicates. *Indicates significant (p < 0.05) differences between groups. Two tail unpaired student’s t test was used for comparison. Black and white dots and white triangles in the graph indicate individual values. Error bars represented as SEM. c Bright-field (BF) image of an SCNT zygote featuring a single PN and a PB (arrows) following activation with electroporation+roscovitine. Scale bar 100 μm. Note that unfertilized but artificially activated SCNT zygotes predominantly display a single pronucleus (1 PN). In contrast, activated and sperm fertilized SCNT zygotes contain two pronuclei originating from somatic and sperm genomes. Results observed in 7 independent experiments. d Activation and embryo development in control and SCNT oocytes after fertilization supplemented with electroporation+roscovitine activation. Bar plots show the mean of 8 independent experiments. *Indicates significant (p < 0.05) differences between groups. Two tail unpaired student’s t test was used for comparison. Black and white dots in the graph indicate individual values. Error bars represented as SEM. e BF image of preimplantation embryo development following SCNT oocyte fertilization supplemented with electroporation+roscovitine treatment. Left: a zygote with 2PNs and a PB. Middle: a 2-cell embryo. Right: a blastocyst. Scale bar 100 μm. Note: All SCNT zygotes that developed to blastocysts had successfully extruded polar bodies, indicating completion of mitomeiosis.
