Fig. 4: Chromosome segregation and ploidy reduction of nonreplicated somatic genomes in SCNT oocytes following mitomeiosis.
From: Induction of experimental cell division to generate cells with reduced chromosome ploidy
a Percentage of SCNT zygotes with segregated and non-segregated somatic chromosomes (N = 90). b Somatic chromosome counts in individual SCNT zygotes (N = 49). Each dot represents an individual SCNT zygote. Red dots indicate zygotes removed from the subsequent segregation pattern analyzes. More detailed segregation results for each zygote are presented in Supplementary data 1, 2, 3, 4. c Mean number of chromosomes counts in PNs and PBs of SCNT zygotes. Only somatic chromosomes derived from SCNT oocytes (without sperm chromosomes) are analyzed. Black and white dots in the graph indicate individual values. PN (N = 38); PB (N = 25) collected from 14 independent experiments. Error bars represented as SEM. d Mean number of segregated homologous chromosomes in PNs and PBs. Black and white dots in the graph indicate individual values. PN (N = 38); PB (N = 25) collected from 14 independent experiments. Error bars represented as SEM. e Comparison of homolog segregation pattern observed in PNs (N = 38) with random segregation pattern derived by the Monte Carlo simulation. Horizontal lines represent the mean; (p = 0.93, Wilcoxon rank sum test). f Comparation of homolog segregation pattern observed in PBs (N = 25) with random segregation pattern derived by the Monte Carlo simulation. Horizontal lines represent the mean; (p = 0.76, Wilcoxon rank sum test). g No linear relationship was found between chromosome length and homolog segregation frequency; R2 = 0.02. h Random distribution of maternal and paternal homologs to PNs and PBs for all chromosomes except the chromosome 8 that showed preferential segregation of maternal homologs to PNs and paternal homologs to PBs. *Indicates significant (p < 0.05) differences between groups. Chi-square test was used for comparison. i Number of segregated homologs analyzed for each chromosome pair for detecting crossover recombinations. Note: only SCNT zygotes that extrude polar bodies were included for analyses. Note: analysis focuses on somatic-origin chromosomes only; sperm chromosomes in fertilized samples are present but excluded from this segregation analysis to enable aggregation between fertilized and non-fertilized SCNT oocytes.
