Fig. 5: Identifying protein binding partners of NEXN in vascular calcification.
From: NEXN protects against vascular calcification by promoting SERCA2 SUMOylation and stabilization
RNA-sequencing analysis identified differentially expressed genes (DEGs) in human VSMCs following infection with either a control adenovirus (Ad-Ctrl) or an Ad-NEXN adenovirus and subsequent stimulation with calcification medium. a Volcano plot of differential analysis (statistics in DESeq2). b KEGG pathway analysis of DEGs (statistics in clusterProfiler). c Gene Ontology (GO)-Cellular Component (CC) analysis of DEGs (statistics in clusterProfiler). d, e GSEA revealed significant enrichment of pathways related to intracellular calcium ion homeostasis and vascular smooth muscle contraction. f Schematic of constructing a Flag-Nexn transgenic male mouse (n = 2 biological replicates). g Schematic of immunoprecipitation-mass spectrometry analysis of aortic tissue from smooth muscle-specific Flag-Nexn male mice and control male mice (WT). h Volcano plot of immunoprecipitation-mass spectrometry differential analysis from smooth muscle-specific Flag-Nexn male mice and WT male mice (statistics in Limma). i Immunoblotting of the interaction between NEXN and SERCA2 using Co-IP experiments in Ad-GFP-NEXN infected human VSMCs (n = 3 biological replicates). The graphic (f) was created by Adobe Illustrator 2021. The graphic (g) was created by Adobe Illustrator 2021, with icons from Servier Medical Art, which is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/).
