Fig. 7: NEXN 299-671 aa acts as a functional region in protecting VSMCs from calcification.
From: NEXN protects against vascular calcification by promoting SERCA2 SUMOylation and stabilization
a Schematic representation of the full-length NEXN plasmid and various truncated fragments. b 293 T cells were co-transfected with plasmids expressing GFP-tagged full-length NEXN and its truncated variants, along with a plasmid expressing FLAG-tagged SERCA2. Following GFP pulldown, the interaction between full-length NEXN, its various fragments, and SERCA2 protein was assessed (n = 3 biological replicates). c Alizarin red staining was performed on human VSMCs infected with an adenovirus expressing NEXN 299-671 aa (Ad-299-671) and then cultured for 7 days in either GM or CM. Scale bar: 500 μm. d, e Western blot analysis was performed to quantify the protein expression of the osteogenic markers Collagen I, RUNX2, and BMP2 in human VSMCs infected with Ad-299-671 and then cultured for 7 days in either GM or CM (n = 4 biological replicates; error bar: mean (centre) ± standard deviation; one-way ANOVA and Turkey’s post hoc test were used for comparisons among more than two groups). GM growth medium, CM calcification medium. Source data are provided as a Source Data file.
