Fig. 8: Achievable cooling rates and necessary CPA concentrations for human organ vitrification.
From: Physical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation
a Predicted minimum cooling rate (CR) as a function of volume of various human organs. b Plot of minimum cooling rate vs. LC (blue curve) and minimum vitrifiable CPA concentration in CRF as a function of LC (red curve). LC is calculated for various organs assuming ellipsoidal shape, and minimum cooing rate occurs at the center of geometry. LC, CR, and dimensions of organs can be found in Table S6. Note that to predict cooling rates at different volumes, one should calculate LC and then use the blue plot in B. The experimental test points (x) showing success of M22, EG+Sucrose, and failure of VS55 at various LC (0.5, 1, 3 L volumes) are also plotted in B, validating the first-order approximation.
