Fig. 4: Non-invasive drug screening application of EGOFETs with hiPSC-CMs.

a Baseline recording of the hiPSC-CM monolayers prior to the addition of NIFE. b Current recording trace after administration of 1 µM of NIFE; c and d are recording traces of hiPSC-CMs after the administration of NIFE. e Overlapped AP signals of recording trace (a). f Overlapping the signals from recording trace (b), depicting the dynamic change of the AP morphology after adding NIFE. g and h are overlapped AP signals from recording traces (c) and (d), respectively. Red arrows in (c), (d), (g) and (h) indicate the diastolic oscillations. i Baseline recording of cells before adding E-4031. j Current recording trace after the addition of 0.1 µM of E-4031. k, l Consecutive recordings after adding E-4031 to monitor its effect. Black and red circles in panel (k) mark the alternated AP morphology commonly called EAD-induced-alternans, which indicate clear pro-arrhythmic events. Green arrows in (l) indicate EADs.