Fig. 1: Antigen persists in discrete cell populations within the lymph node.

A Experimental design. Created in BioRender. Tamburini, B. (2025) https://BioRender.com/q5mhs3w. B Mean Ag-score is shown for each cell type for 2, 14, 21, and 42 days post vaccination. For the 21 and 42 day time points one replicate is shown, a second replicate is shown in Figure S3. For each replicate experiment, 13 mice were used per group where popliteal LNs from 9 mice were pooled for CD45- selection and popliteal LNs from 4 mice were pooled for CD45+ cell enrichment of myeloid cell populations prior to sequencing. C UMAP projection shows LEC subsets sequenced after CD45- enrichment of 18 popliteal LNs from 9 mice. D UMAP projections show Ag-scores for LEC subsets for each timepoint, replicates described as in (B). E Mean Ag-score is shown for LEC subsets for each timepoint. Replicates as described in (B). F Ag-scores are shown for each timepoint for each LEC subset. The number of cells plotted is shown above each boxplot. The center line, box limits, whiskers, and points represent the median, interquartile range (IQR), the range within 1.5×IQR from the box limits, and points outside this range (outliers), respectively. One biological replicate is shown as described in (B). G UMAP projection shows DC subsets from 8 popliteal LNs per group combined and sequenced after sorting shown in Fig. S1A where CD11c + , CD11b + , B220+ and “other” cells were recombined at a 4:4:1:1 ratio post sorting. One replicate is shown. A second replicate is show in Figure S3. H UMAP projections show Ag-scores for DC subsets for each timepoint, replicates described as in (B, F). I Mean Ag-score is shown for DC subsets for each timepoint as described in (E). J Ag-scores are shown for each timepoint for each DC subset as described in (F).