Fig. 5: Antigen exchange with DCs correlates with levels of antigen archiving.

A Antigen counts are shown for adjacent LN tissue sections from a dual-immunized mouse analyzed with the Xenium platform. B Localization of Ag+ and Ag- cells is shown for LECs for tissue sections from (A). C Localization of Ag+ and Ag- cells is shown for DCs for tissue sections from (A). D Cell-cell interaction enrichment results are shown for section 1 from A for Ag + /- LECs and DCs. Red bars indicate LEC-DC interactions, arrows indicate significant enrichment for LEC-DC interactions, interactions between other cell types are shown as grey bars. E The distance to the closest Ag+ LEC is shown for Ag+ and Ag- DCs for section 1 from (A). F Mean day 21 (BC2) Ag-score is shown for DCs from mice that received a single immunization (day 21-Group A) or dual immunization (day 0 and day 21-Group C). G Ag-score is shown for single and dual immunizations for the 21 day timepoint (BC2) for DC subsets with the highest Ag-score. Other timepoints are shown in grey. P-values were calculated using a one-sided Wilcoxon rank sum test with Benjamini-Hochberg correction. Values are shown for one biological replicate, a second biological replicate is shown in Figure S8. Each “biological replicate” contains cells from 8 mouse LNs pooled. The center line, box limits, whiskers, and points represent the median, interquartile range (IQR), the range within 1.5×IQR from the box limits, and points outside this range (outliers), respectively. H Mean day 0 (BC1) Ag-score is shown for DCs from mice that received single or dual immunization as described in (F) except for Group B and C. I Ag-score is shown for single and dual immunizations for the day 0 timepoint as described in (G) except for BC1.