Fig. 5: GM-CSF signal is required for CD301b⁺ cDC2 development.

Frequencies of cDC1s and cDC2 subsets in mouse lungs at steady state (a–c) and after OVA/HDE inhalation (d) Representative cytograms (left panels) and compiled data (right panels) of Ly6C^– cDC2s are shown (a–c), n = 3 biological replicates; d, C57BL/6 n = 3, Csf2rb^fx n = 7, Csf2rb^∆DC n = 8 biological replicates). e Treg induction by lung cDC2s. Flow cytometric analysis of CD25⁺ and Foxp3⁺ Tregs after culture of naïve CD4⁺ T cells from Foxp3^eGFP OT-II mice with or without total lung cDC2s from mice that received OVA (n = 4 technical replicates). f Effector T cell responses induced by lung cDC2s. Proliferation and cytokine production by T cells, as determined by cell count and ELISA, respectively, after the culture of naïve CD4⁺ T cells from OT-II mice with or without total lung cDC2s mice that received OVA/HDE (n = 3 technical replicates). a–d Data were analyzed by ordinary one-way ANOVA with Tukey’s multiple comparison test. e, f Data were analyzed by two-tailed unpaired t-test. (a–d) Each dot represents an individual mouse. e, f Each dot represents separately cultured CD4⁺ T cells. a–c, e, f Representative results from two experiments (d) combined results from two experiments. Comparison between Csf2rb^fx and Csf2rb^∆DC cDC2s are shown. Data are presented as mean values ± SEM. P values are indicated above the graphs. Source data are provided as a Source Data file. GM-CSF granulocyte macrophage colony stimulating factor, cDC, conventional dendritic cells, OVA ovalbumin, HDE house dust extract, Treg regulatory CD4⁺ T cells, ELISA enzyme-linked immunosorbent assay.