Fig. 1: Human iPSC-derived neurons trigger antigen-dependent CD8+ T cell activation. | Nature Communications

Fig. 1: Human iPSC-derived neurons trigger antigen-dependent CD8+ T cell activation.

From: Neuron-reactive KIR+CD8+ T cells display an encephalitogenic transcriptional program in autoimmune encephalitis

Fig. 1

a Representative fluorescence microscopy images of hiPSC-derived neurons from HD6, immunostained for NF-200 (magenta), HLA-ABC (yellow) and DAPI (blue), untreated (left panels, control) or treated with IFNγ and TNFα for 48 h (right panels, IFNγ + TNFα). Bar represents 100 μm. b Flow cytometry analysis of HLA class I expression (anti-HLA-ABC antibody (clone W6/32)) of hiPSC-derived neurons from 6 healthy donors (HD1-HD6), untreated (control) or treated with IFNγ and TNFα for 48 h (two-sided Wilcoxon test, p value = 0.0156). n = 6 biological replicates (i.e., donors), in one experiment. c Correlation of the number of spot-forming units (SFU)/million PBMCs in an IFNγ ELISpot assay (y-axis) versus the percentage of IFNγ+CD8+ T cells as measured by IFNγ secretion assay (x-axis). In both assays, PBMC (ELISpot) or neurons (IFNγ secretion assay) were pulsed overnight with a pool of CD8+ T cell-restricted immunodominant viral epitopes from EBV, CMV and VZV. Correlations were assessed by running a two-sided Pearson correlation test. n = 9 biological replicates (3 peptide pools per 3 donors), in one experiment.

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