Fig. 7: SERF2 regulates stress granule formation and dynamics.
From: Visualization of liquid-liquid phase transitions using a tiny G-quadruplex binding protein
a Immunofluorescence of SERF2 and G3BP1 in fixed U2OS cells treated with 0.5 mM sodium arsenite for 1 h. b Plot shows the percentage of stress granule-positive cells after sodium arsenite treatment calculated from images shown in (a). Error bars were calculated from four independent experiments, and data are presented as mean values. c Live-cell imaging of EGFP-FUS HeLa Kyoto cells treated either with a control RNA (siCTRL) or an RNA targeting SERF (siSERF2), treated with different stressors (0.5 mM Sodium arsenite, 0.4 M Sorbitol, or 10 µM MG132) for 1 h. Scale bars in (a and c) are 10 µm. d Plot shows percentage of stress granule-positive cells under different stress treatments calculated from images shown in (c). At least 50 cells from three biological replicates were analyzed, and data are presented as mean values. **** shown in (b and d) indicates P < 0.0001. e, f These graphs show FRAP recovery curves in EGFP-FUS HeLa Kyoto live cells with siCTRL or siSERF2 conditions treated with 0.4 M sorbitol (e) and 0.5 mM sodium arsenite (f). Standard deviations were calculated by analyzing four different foci in three replicates subjected to FRAP, and data are presented as mean values.
