Fig. 2: Spatial atlas of mammary fibroblasts during pubertal morphogenesis.
From: Contractile fibroblasts form a transient niche for the branching mammary epithelium
a A dot plot showing expression of marker genes used for spatial mapping. b A representative overview of mammary gland on a histological section: red squares show fields of view (FOV) containing ducts, TEBs or distal fat pad. Scale bar: 100 µm. Representative of >5 biological replicates. c Representative images showing the expression of various RNA or protein markers in regions containing ducts, TEBs or distal fat pad, the insets show details on fibroblasts. * marks the epithelial compartment encircled by a yellow dashed line. Scale bar: 100 µm, 10 µm in detail. d Quantification of marker-positive stromal cells in different regions of the mammary gland, shown as box plots. Each dot represents a single FOV, n = 3 biological replicates, N = 59 FOVs for αSMA, 57 FOVs for SDC1, 113 FOVs for Mfap4, 90 FOVs for Pi16, 74 FOVs for F3, 81 FOVs for Enpep. Statistical analysis: Wilcoxon test, two-sided.
