Fig. 2: Construction of ABEs targeting the Pou4f3Q113* mutation site.
From: Optimized in vivo base editing restores auditory function in a DFNA15 mouse model
A Structural diagram of the ABE base editors, consisting of TadA deaminase and nCas9. The red color represents the subunit that varied, with nCas9 (above) as the variable, and TadA (below) as the variable. The sgRNA is in green, and the dsDNA is in black. A·T is the target base pair. B Schematic of the Pou4f3 locus highlighting the c.337C > T mutation and the five candidate sgRNAs that the position of target A (in red) in the protospacer (6, 7, 12, 4, and 5) and that use different PAMs (CGGG, GCGG, GTGA, GGG, and CGG, in blue). C The heat maps represent the A-to-G transition frequency of the 12 ABEs at five targets (sgRNA1-5) in the HEK293T-Pou4f3Q113* cell lines. Data were collected from three independent experiments. D The frequencies of indel formation by twelve ABEs at five target sites in (A, B), corresponding to NGS data analysis. Data are represented as mean ± SEM from three independent experiments. E The precision of A-to-G base editing at the sgRNA3 by SchABE8e. A-to-G (red); “correction”: no bystanders. Source data are provided as a Source data file.
