Fig. 2: Cryo-EM structure of SLCO2A1.
From: Structure and transport mechanism of the human prostaglandin transporter SLCO2A1
a, b The EM map of SLCO2A1-apo embedded in LMNG micelle (gray) (a) and the cartoon representation of SLCO2A1-apo (b). The N and C domain of SLCO2A1-apo are colored in green and pink, respectively. c Topology diagram of human SLCO2A1 from N-terminal to the C-terminal. Unsolved regions are shown with dashed lines. d Multiple disulfide bonds within EL9–10 are shown as yellow sticks. A fragment of EL3–4 is attached to EL9–10 via a disulfide bond. e 6-CF transport by HEK293F cells expressing SLCO2A1 WT or SLCO2A1 mutants. Cells expressing mCherry served as a control. Data were normalized to SLCO2A1 WT and are presented as mean ± s.e.m.; n = 3 independent replicates for mutants. n = 6 independent replicates for mCherry and SLCO2A1 WT. One-way ANOVA was used; ****P < 0.0001. f A cut-open electrostatic potential surface of SLCO2A1-apo. The electrostatic potential surface was calculated in PyMoL (red to blue, −50 kT/e− to +50 kT/e−). Red arrow indicates the central pocket of SLCO2A1. Source Data are provided as a Source Data file.
