Fig. 5: Understanding the improved performance of CLpATL.
From: Genetic controllers for enhancing the evolutionary longevity of synthetic gene circuits in bacteria
a–c Comparing CLpATX (blue) and CLpATL (red). a Population-wide process outputs P over time for two representative controllers. b Initial output P0 against the maximal transcription rate of the process gene ωA across all optimal controllers. c Controller protein per cell pB vs process output protein per cell pA across all optimal controllers. d–f The effect of controller mutation on CLpATL with nB = 300 aa. d Population-wide output P over time according to mutation state. e Population size according to mutation state. f Bar charts for CLpATL with nB = 300 aa showing (i) growth rate λ (min−1), (ii) process protein output per cell pA (molecules/cell) and (iii) sRNA per cell rC (molecules/cell). Each subplot represents a unique mutation state. Moving rightwards signifies mutation of the process, and moving downwards signifies mutation of the controller. Simulation results are provided as a Source Data file.
