Fig. 6: Optimal outputs for CLλTL.
From: Genetic controllers for enhancing the evolutionary longevity of synthetic gene circuits in bacteria
a A schematic describing the controller. Controller protein pB is produced from a growth-sensitive promoter and activates the production of sRNA rC. rC combines with and deactivates process mRNA mA. See key in Fig. 2a for symbol meanings. b–e Optimal performance for both CLλTL (orange) and CLpATL (red) for controller protein length nB = 1, 300, 600 aa. b τ±10 vs initial output P0, (c) τ50 vs initial output P0, (d) %-change in τ±10 over open-loop vs initial output P0, (e) %-change in τ50 over open-loop vs initial output P0. f–h Robustness analysis. For each of the 100 optimal controllers with nB = 300 aa, 100 further controllers were generated by varying parameters by up to ± 10% (dark grey) and ± 25% (light grey). The percentage changes in three output metrics were calculated versus the original optimal systems: (f) P0, (g) τ±10 and (h) τ50. Plots show the means (for ± 10%) and standard deviations (for both ± 10% and ± 25%) of the percentage changes for each optimal controller. Percentages marked on the plots indicate the standard deviations across the entire Pareto front when parameters were varied by ± 10%(± 25%). Only original optimal controllers where τ±10 = τ90 are considered. Simulation results are provided as a Source Data file.
