Fig. 3: BcSSP2 interacts with NUP62 and partitions into NUP62 condensates. | Nature Communications

Fig. 3: BcSSP2 interacts with NUP62 and partitions into NUP62 condensates.

From: The pathogen effector BcSSP2 suppresses the NPC phase separation to facilitate Botrytis cinerea infection

Fig. 3

a–d BcSSP2 interacts with NUP62. a Luciferase activity was detected at 36 hpi in the LCI assay. b Flag-NUP62 and BcSSP2-GFP were transiently expressed in tobacco leaves and immunoprecipitated with GFP trap in the Co-IP assay. Total extracts (Input) and the immunoprecipitates (IP) were analyzed by immunoblots. c, d Immobilized GST-NUP62, GST-NUP62-NTD and GST-NUP62-CTD were used to pull down BcSSP2-His. TrxA-His (control-His) serves as a negative control for BcSSP2-His. Ponceau S staining indicates the loading of bait proteins. e BcSSP2 partitions into NUP62 condensates. Confocal image of NUP62-Alexa 546 with BcSSP2-Alexa 488 (TrxA-Alexa 488 serves as a control) and fluorescent intensity along the arrows are shown, respectively. Scale bar: 5 μm. Source data are provided as a Source Data file.

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