Fig. 1: Heterologous expression of tardigrade Dsup in budding yeast promotes survival after chronic exposure to an oxidative DNA damaging agent, reduces related DNA damage, and extends lifespan through chronic endogenous oxidative damage. | Nature Communications

Fig. 1: Heterologous expression of tardigrade Dsup in budding yeast promotes survival after chronic exposure to an oxidative DNA damaging agent, reduces related DNA damage, and extends lifespan through chronic endogenous oxidative damage.

From: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage

Fig. 1

a Immunoblot of Dsup-FLAG (from pTDH3-6His-Dsup-FLAG) and H2B-FLAG in yeast strains containing a single integrated copy of each tagged gene (Supplementary Data File 1B). The same protein samples were ran on a second gel for detection of GAPDH and H2B. Three dilutions of protein extracts loaded as indicated. EV, Empty vector. b Relative sensitivity of yeast strains (five-fold serial dilutions) to indicated doses of hydrogen peroxide (H2O2), methyl methanesulfonate (MMS), Zeocin (Zeo), or UV. Yap1∆ is a positive control for sensitivity to oxidative DNA damage (H2O2). Cac1∆rtt106∆ is a positive control for sensitivity to other genotoxins. WT (BY4741), wild-type yeast. Dsup (WT), Dsup1 (aa 1-455). c Yeast cells expressing Dsup (WT) show reduced levels of 8-hydroxy 2 deoxyguanosine (8-OhdG) in response to oxidative DNA damage (120 min in 10 mM H2O2; mean and standard deviation from three independent experiments; p-value determined using a two-sided Student’s t-test). d. Yeast cells expressing Dsup (WT) show increased replicative lifespan when exposed to chronic oxidative damage (sod1∆−/+ Dsup (WT); n = 30 individuals for each background). Source data are provided as a Source Data file.

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