Fig. 3: Dsup promotes survival after oxidative DNA damage in a manner that requires its C-terminus but is not due to ROS scavenging. | Nature Communications

Fig. 3: Dsup promotes survival after oxidative DNA damage in a manner that requires its C-terminus but is not due to ROS scavenging.

From: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage

Fig. 3

a The Dsup HMGN-like motif (aa 363-370) and C-terminal region (aa 371-445) are required to confer yeast resistance to chronic H2O2 -mediated oxidative damage (concentrations indicated). yap1∆ is a positive control for sensitivity to H2O2. WT, wild-type. EV, Empty vector. b The Dsup HMGN-like motif and C-terminal region are required to confer yeast resistance to acute H2O2 -mediated oxidative damage. Graph plots percentage cell survival after 90-minute exposure to H2O2 (concentrations indicated). Shown are average and standard deviation of experiments using three independent colonies for each strain. p-values were determined using two-sided Student’s t-test. c Relative redox state in the cytoplasm (left; roGFP2-Grx1 [redox sensitive GFP - GlutaRedoXin 1] reporter) and nucleus (right; roGFP2-Grx1-NLS reporter) for indicated yeast strains (color key as in b) through H2O2 exposure (4 mM; time in min). T0 sample was taken immediately after H2O2 addition. Shown are average and standard deviation of experiments performed from three independent colonies. Source data are provided as a Source Data file.

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