Fig. 5: Dsup expressing yeast do not show a poised or elevated transcriptional response to oxidative stress. | Nature Communications

Fig. 5: Dsup expressing yeast do not show a poised or elevated transcriptional response to oxidative stress.

From: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage

Fig. 5

Yeast +/− Dsup were exposed to H2O2 (0, 4 or 8 mM for 30 min; T0 is untreated) and RNA-seq performed. Indicated comparisons show differentially expressed genes (Supplementary Data File 3) from the most significantly enriched functional annotation categories and their false discovery rates (FDR; as determined by DAVID). Group 2 Dsup-upregulated genes included a set involved in amino acid biosynthesis. However, this did not extend to activation of the ‘General Amino Acid Response’, including elevated protein levels of Gcn4 transcriptional activator80 (Supplementary Fig. 5). Data are the average of three independent repeats. EV, Empty vector. Source data are provided as a Source Data file.

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