Fig. 7: The Dsup HMGN-like motif and C-terminal region each contribute to yeast survival during oxidative damage. | Nature Communications

Fig. 7: The Dsup HMGN-like motif and C-terminal region each contribute to yeast survival during oxidative damage.

From: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage

Fig. 7

a Immunoblot shows relative expression of Dsup alleles (anti-FLAG) that delete or mutate (-3R/3E) the HMGN-like motif (aa 363-370) and/or C-terminal region (aa 372-455) in yeast (each N-terminal 6xHIS and C-terminal FLAG tagged; see also Fig. 2a–d). Anti-GAPDH is a loading control resolved on the same gel. EV, Empty vector. Dsup (WT), (aa 1-455). b Relative sensitivity of yeast strains (five-fold serial dilutions) to oxidative DNA damage by H2O2 (see also Figs. 1b and 3a). yap1∆ is a positive control for sensitivity to H2O2. WT (BY4741), wild-type yeast. Source data are provided as a Source Data file.

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