Fig. 8: Dsup binds nucleosomal DNA, histone tails and the acidic patch. | Nature Communications

Fig. 8: Dsup binds nucleosomal DNA, histone tails and the acidic patch.

From: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage

Fig. 8

Relative interaction of Dsup (WT) with fully defined nucleosomes (Supplementary Data File 1d) containing histone tail truncations (a), lysine acetylations (b), lysine methylations (c), or acid patch mutations (d). All assays performed under optimized conditions (from Fig. 6b): Dsup (WT: 1 - 0 μM in two-fold serial dilutions), nucleosome (each as indicated; 10 nM), free DNA (147 × 601; 2.5 nM), 150 mM NaCl, 1 mg/ml salDNA. Data are the average and standard deviation of two technical repeats. Source data are provided as a Source Data file.

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