Fig. 7: Repression of circMAN1A2(2,3,4,5) induces chromosome instability via CENPB mRNA-level modulation.

a, b KD of circMAN1A2(2,3,4,5) led to binuclei or multinuclei (a) and aneuploidy formation (b) in circMAN1A2(2,3,4,5)-KD HT29 and DLD-1 cells. Top, representative images of nuclei (a) and chromosomes (b), as revealed by immunofluorescent (IF) staining of F-actin (red), nuclei (blue), and chromosomes (blue). Bottom, statistics of abnormal cells percentage in interphase (n = 60 cells/independent experiments) (a) and chromosome numbers per cell (n = 60 cells) (b). c KD of circMAN1A2(2,3,4,5) resulted in a marked reduction of cells in S phase and a pronounced accumulation in G2/M phase, as revealed by cell cycle analysis. d, e Over-expression of CENPB rescued the cell cycle (d) and proliferation (e) deficiency with circMAN1A2(2,3,4,5) KD, as revealed by cell cycle analysis (d) and MTT cell proliferation assay (e; n = 5 wells of cells examined over three independent experiments)). Data are presented as mean ± s.d. (a, c–e), with biologically individual data points shown (a, c, d). P values were determined by ordinary one-way ANOVA test with Dunnett’s post-hoc comparisons versus scramble control (FDR-adjusted P values) (a) and ordinary two-way ANOVA with Dunnett’s post-hoc comparisons versus scramble control at each row factor level (adjusted P values) (c–e). Source data are provided as a Source Data file.