Fig. 3: Ace-Resi remodels the brain immune environment. | Nature Communications

Fig. 3: Ace-Resi remodels the brain immune environment.

From: Post-resection delivery of a TLR7/8 agonist from a biodegradable scaffold achieves immune-mediated glioblastoma clearance and protection against tumor challenge in mice

Fig. 3

A Timeline of study design. CT2A-Luc tumors were implanted 2 weeks prior; mice were randomized to treatment group. Mice were euthanized days 1, 3, 7, and 10 after treatment and organs harvested for flow analysis. Healthy mice were included as a control. B UMAP of 9 leukocyte populations in the brain on Day 1, with corresponding heatmap of TLR7 expression. n = 5 healthy, n = 3 tumor, n = 3 Ace-Blank, and n = 5 Ace-Resi mice, one independent experiment. C Mean fluorescence intensity (MFI) of CD86 and CD80 in microglia on Day 1. n = 5 healthy, n = 3 tumor, n = 3 Ace-Blank, and n = 5 Ace-Resi mice, one independent experiment. Data are presented as mean ± SD. P-value, one-way ANOVA, Tukey’s multiple comparisons test. D MFI of CD80 in monocytes on Day 1. n = 5 healthy, n = 3 tumor, n = 3 Ace-Blank, and n = 5 Ace-Resi mice, one independent experiment. Data are presented as mean ± SD. P-value, one-way ANOVA, Tukey’s multiple comparisons test. E MFI of CD80 on Day 1 and CD206 on Days 3 and 7 in tumor associated macrophages (TAM). n = 5 healthy, n = 3 tumor, n = 3 Ace-Blank, and n = 5 Ace-Resi mice, one independent experiment. Data are presented as mean ± SD. P-value, one-way ANOVA, Tukey’s multiple comparisons test. F MFI of CD86 and CD80 in conventional dendritic cells (cDC) on Day 1. n = 5 healthy, n = 3 tumor, n = 3 Ace-Blank, and n = 5 Ace-Resi mice, one independent experiment. Data are presented as mean ± SD. P-value, one-way ANOVA, Tukey’s multiple comparisons test.

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