Fig. 2: Intrinsic expression of blaTEM-1 shapes co-amoxiclav MIC across the E. coli phylogeny.

a A midpoint-rooted core gene phylogeny of E. coli chromosomes from the 67 isolates with expression quantified. Tips are coloured by phylogroup. ST12, ST127 and ST69 are highlighted with tip outlines and circles at their crown nodes. Tip shape distinguishes location of blaTEM-1 on chromosomes (circles) and plasmids (squares). b Bar length records mean blaTEM-1 ĪCt for each tip (log10-scaled; lower values denote higher blaTEM-1 expression). Bar colour records isolate co-amoxiclav MIC for each tip. c Posterior means (coloured circles) and 95% HPD intervals (horizontal lines) for phylogenetic effect on negative blaTEM-1 ĪCt for each tip (multiplied by ā1 for ease of comparison). Red indicates above average expression and blue indicates below average expression. d Posterior means (coloured circles) and 95% HPD intervals (horizontal lines) for phylogenetic effect on co-amoxiclav MIC for each tip. Pink indicates above average MIC and green indicates below average MIC. Note that isolates OXEC-238 and OXEC-490 were excluded from the MIC model (see exclusion criteria).