Fig. 6: DF-003 suppresses pro-inflammatory gene upregulation in the retina, optic nerve, and brain cortex of ROSAH model mice.
From: Discovery of a selective alpha-kinase 1 inhibitor for the rare genetic disease ROSAH syndrome
Female hALPK1-KI and hALPK1[T237M]-KI mice were treated orally with DF-003 once per day for 10 days at the indicated doses (n = 8 animals for each group). The following day, animals were euthanized and the mRNA levels of the indicated genes in the retina, optic nerve, and brain cortex samples from these mice were analyzed by qPCR and normalized to levels of Rpl13. Data represent means ± SEM for each genetic and treatment group, with each dot representing an organ sample from one animal. In some analyses, group sizes were <8 due to RNA sample loss during in vitro procedures or due to the detection limit. Statistical outliers were identified using Grubb’s test. All excluded data points were indicated in the Source Data file. Statistical comparisons between vehicle groups for the two genotypes were made using two-tailed unpaired Student’s t-tests, and comparisons between DF-003-treated groups to the vehicle group within the same genotype were made with one-way ANOVAs followed by Sidak’s post hoc tests. p-values <0.05 have been indicated on the charts.
