Fig. 2: Synergistic development of functional components in motor assembloids. | Nature Communications

Fig. 2: Synergistic development of functional components in motor assembloids.

From: Geometrically-engineered human motor assembloids-on-a-chip for neuromuscular interaction readout and hypoxia-driven disease modeling

Fig. 2

a Schematic illustrating experimental setups for motor assembloids to explore interactions between hSkM and hMNS. b and c Representative western blots (b) and heatmap profiles (c) showing the protein levels of MHC, MHC-I, MHC-II, sarcomeric α-actinin (SAA) and myosin light chain (MYL) in organoids with or without hMNS over 14 days. with β-tubulin as loading control. n = 3 independent experiments. d Gene expression (RT-qPCR) of myosin complex-related markers over 14 days in organoids with or without hMNS, normalized to 18S. n = 3 independent experiments. e Relative gene expression of motor neuron subtype-related markers in organoids with or without hSkM over 14 days. n = 3 independent experiments. f Schematic of the model of non-innervated hSkM organoids (n = 3 organoids) and hSkM-hMNS assembloids (n = 4 assembloids), with a corresponding volcano plot showing differential gene expression from bulk RNA-seq. g Heatmap with gene ontology (GO) enrichment annotations illustrating differential gene expression and functional categorization in non-innervated organoids and motor assembloids. Data are presented as mean ± SD. Statistical analysis: e two-tailed Student’s t test. b, d Two-way ANOVA with Bonferroni multiple comparison. ***p  <  0.001, **p <  0.01, *p  <  0.05. Source data are provided as a Source Data file.

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