Fig. 6: Effects of TP63 overexpression on gene expression, chromatin accessibility and chromatin structure. | Nature Communications

Fig. 6: Effects of TP63 overexpression on gene expression, chromatin accessibility and chromatin structure.

From: TP63 mediates the generation of tumour-specific chromatin loops that underlie MYC activation in radiation-induced tumorigenesis

Fig. 6

A MYC expression difference before and after TP63 overexpression. pValue is calculated using DEseq2. B Western blot of MYC before and after TP63 overexpression. The experiment was repeated three times independently with similar results. Uncropped and unprocessed scans of all blots are provided as a Source Data file. C Venn diagram shows the overlap between BERP35T1 up-regulated genes and BEP2D OE up-regulated genes. D TP63 CUT&Tag, ATAC-seq, CTCF CUT&Tag and Cohesin CUT&Tag heatmap before and after TP63 overexpression. According to ATCA-seq signal foldchange, TP63 peaks were divided into 4 groups, including strongly increase (foldchange≥4), slightly increase (2≤foldchange<4), not significant (0.5<foldchange<2) and decrease (foldchange≤0.5). E APA of contact mediated by different group of TP63 peaks. F Gene expression foldchange mediated by different group of TP63 peaks. Box limits represent 25th percentile, median and 75th percentile. Whiskers represent 5th and 95th percentile. Points represent outliers beyond whiskers. Significance: **P < 0.01, two-sided Wilcox-test. Source data are provided as a Source Data file.

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