Fig. 5: General modulation effects of AAs and the transferability to short homopeptides.

a NPM1 concentration in the dilute phase after the addition of different AAs (200 mM of S, T, Q, C, G, P, A, V; 100 mM of H, N, I, L, F; 20 mM K, E) divided by reference NPM1 concentration in the dilute phase in the absence of any AAs; b K72 concentration in the dilute phase after the addition of different AAs (200 mM of S, T, Q, G, P, A, V; 100 mM of M, H, N, I, L, F; 20 mM K, E) divided by reference K72 concentration in the dilute phase in the absence of any AA; AAs are groups in colours by side chain properties. Orange, green, blue, and purple bars represent polar uncharged, nonpolar aliphatic, nonpolar aromatic, and slightly positively charged side groups, respectively; c, d NPM1 concentration changes in the dilute and condensate phase after the addition of G and (G)₃ at the same ionic strength; e, f NPM1 concentration changes in the dilute and condensate phases after the addition of P and (P)₃ at the same ionic strength. Data are expressed as mean ± standard deviation of n = 3 independent experiments.