Fig. 4: Model of ASAP1 PH:myrArf1 complex at the membrane surface.
From: An active allosteric mechanism in ASAP1-mediated Arf1 GTP hydrolysis redefines PH domain function
A Snapshot representative of the model of the complex between myrArf1 (blue ribbon) with the GTP nucleotide represented as ball and stick and ASAP1 PH (gold ribbon) at the membrane surface. B ASAP1 PH: Arf interaction counts plotted against residue number and plotted on the model. Data were averaged over all MD trajectories with a cutoff of 3.5 Å to detect nonhydrogen proximities. C Key interactions at the interface. Stable intermolecular salt bridges and residues forming the hydrophobic cluster comprising Phe51, Trp66, Ile74, Leu77 and Trp78 on Arf1 and Leu 386 (β4/β5 loop), His405 (β6/β7 loop) and Leu 434, Ala 437 and Phe438 (end of the C-terminal helix) on ASAP1 PH are shown. All images created using Chimera56. Source data are provided as a Source data file.
