Fig. 3: Structural comparison of DNA bound forms of MET1 and DNMT1.
a Superimposition of the structure of MET1MTase bound to hmDNA (yellow) on that of DNMT1 bound to hmDNA and H3ub2 (cyan, PDB: 7XI9). The magnified figures on the left show the TRD structures in which the upper and lower panels are DNMT1 and MET1, respectively. The magnified figures on the right display the structures of the BAH2 loop of DNMT1 (upper panel) and MET1 (lower panel). b DNA methyltransferase activity of MET1 mutants in the MTase domain. Data are presented as the mean ± SD for n = 3 independent biological replicates. c DNA methyltransferase activity of MET1 mutants in Activating Helix. Data are presented as the mean ± SD for n = 3 independent biological replicates. d Structural model of G1101S mutation. SAH and Gly1101 in the left panel are depicted as gray and yellow sticks superimposed on the cryo-EM map, respectively. The right panel presents the Ser1101 substitution model. e Structure around P1300 of the MTase domain of MET1. P1300 and the residues of catalytic core are shown as yellow and orange stick transparent sphere, respectively.
