Fig. 2: Multiplexed detection of 30 RNAs and two conjugated antibodies.
From: Cassini: streamlined and scalable method for in situ profiling of RNA and protein
a All 30 RNA types are displayed in a mouse brain hemisphere, with rectangles indicating zoomed-in areas shown in (c) and (d). b Four marker genes of the hippocampus. c Six markers indicating the cortical layers and d six neuronal markers, along with the corresponding density. In c and d, the density of the markers within the stripe indicated at the top has been calculated using the Epanechnikov kernel. e immunostaining (black) and the corresponding RNA (red) of GFAP and NeuN.
