Fig. 2: Spermatogenesis and sperm morphology, and motility in Het and KO males of Galntl5 variants 1 and 2 are normal. | Nature Communications

Fig. 2: Spermatogenesis and sperm morphology, and motility in Het and KO males of Galntl5 variants 1 and 2 are normal.

From: GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding

Fig. 2

a Testis morphology. b Testis weight. There was no difference in the testis weight between Galntl5 Het [3.15 ± 0.56 (em2/wt)] and KO mice [3.2 ± 1.1 (em2/em2)] (Mann–Whitney test, p = 0.94). ns: not significant. c Histological analysis of testes with Periodic Acid Schiff (PAS)-Hematoxylin staining. The area dotted by the yellow color was magnified (right panels). The data reproducibility was checked by two biological replicates. d Sperm morphology. The microscopic observation of sperm from Galntl5 Het and KO mice was comparable to WT sperm. The data reproducibility was checked by two biological replicates. e Sperm motility analysis with CASA. After 10- and 120-min incubation, each parameter of the sperm motility was measured. There were no differences among the groups [Kruskal–Wallis test, sperm motility: p = 0.99 (10 min), and p = 0.55 (2 h), sperm progressive motility: p = 0.83 (10 min), and p = 0.50 (2 h)].

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