Fig. 3: H2O2-mediated OsPHR2 Cys377 oxidation is critical for promoting Pi-starvation responses in rice. | Nature Communications

Fig. 3: H2O2-mediated OsPHR2 Cys377 oxidation is critical for promoting Pi-starvation responses in rice.

From: H2O2-mediated oxidation of PHOSPHATE STARVATION RESPONSE2 promotes adaptation to low phosphate in rice

Fig. 3

a In vitro analysis of the oxidation of OsPHR2 and its mutated variants. The MBP-OsPHR2 and its mutated variants pretreated with 0 or 5 mM H2O2 for 30 min were subjected to biotin switch assay using anti-MBP antibody. b Analysis of oxidation of OsPHR2 and its mutated variants in rice. Total proteins were extracted from 7-day-old OsPHR2-GFP/phr2, C140S-GFP/phr2, and C377S-GFP/phr2 plants treated with 2 mM H2O2 or LP for 2 days and then subjected to biotin switch assay using anti-GFP antibody. c–f The morphology (c), biomass (d), Pi concentrations in shoot (e) and root (f) of wild type XS134, phr2, OsPHR2-GFP/phr2, C140S-GFP/phr2, and C377S-GFP/phr2 plants cultured hydroponically under HP or LP conditions for 28 days. Bar = 10 cm in c; DW dry weight, FW fresh weight. The immunoblot analysis was repeated three times with similar results for a–c. Data are means ± SD (n  =  3 replicates, 10 seedlings per replicate for d; n  =  5 replicates, 3 seedlings per replicate for e and f). The different letters above the bars denote significant differences (P  <  0.05) according to a two-sided Duncan’s multiple range test for d–f. Source data are provided in the Source Data file.

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