Fig. 2: ICE and IncF components interact to form hybrid IMC and OMCC. | Nature Communications

Fig. 2: ICE and IncF components interact to form hybrid IMC and OMCC.

From: The assembly of a hybrid type IV secretion system by a Crohn’s disease-associated Escherichia coli strain

Fig. 2

A Purification of putative TraCICE using affinity followed by size exclusion chromatography. B Purified TraCICE shows ATPase activity in vitro. Data represent three independent replicates and are presented as mean values +/− SEM. C Co-purification of TraCICE and TraLIncF using affinity followed by size-exclusion chromatography. D Co-purification of the hybrid OMCC from E. coli co-expressing TraBHIncF, TraKIncF, and TraVICE (~17 kDa). An HA-tag was added to the C-terminus of TraVICE to confirm its identity via immunoblotting. Three independent experiments were performed, and similar results were obtained every time. Source data are provided as a Source Data file.

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