Fig. 6: The Hi-C analysis indicates that Lemd3 depletion changes inter-TAD interactions. | Nature Communications

Fig. 6: The Hi-C analysis indicates that Lemd3 depletion changes inter-TAD interactions.

From: The inner nuclear membrane protein LEMD3 organizes the 3D chromatin architecture to maintain vascular smooth muscle cell identity

Fig. 6

a Heatmap showing the alterations in long-range compartment interactions in the region of 50–100 Mb on chromosome 8 upon Lemd3 depletion. The red box indicates increased interactions between A-B compartments, and the black box indicates decreased interactions between B-B compartments. The bin size is 100 kb. b Bar plots showing the percent change in TAD-TAD interactions. c Box plot showing the average contacts of adjacent and non-adjacent TAD pairs. Box plots are defined in terms of the minimum values and maximum values (whiskers), median (center line) and 25th and 75th percentiles (bounds of box). Adjacent: n = 4323 TAD pairs; Non-adjacent: n = 259868 TAD pairs. d Contour plots showing the distribution of adjacent TAD pairs with decreased or increased interactions upon Lemd3 depletion. e Histogram showing the proportion of adjacent TAD pairs with increased or decreased interactions upon Lemd3 depletion. Heatmap showing the alterations in adjacent TAD-TAD interactions at the A-B boundary in the region of 21‒24 Mb on chromosome 6 (f) and the region of 74‒77 Mb on chromosome 11 (g) upon Lemd3 depletion. The dotted boxes denote inter-TAD interactions at the A-B boundary. The bin size is 40 kb. Statistical analysis was performed using the Mann‒Whitney U test for the data in (c).

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