Fig. 1: AdhE and BdhE possessing similar yet evolutionarily independent domain architectures.
From: Repeatability of protein structural evolution following convergent gene fusions
a Histogram of maximum alignment identities (%) of bacterial proteins hit by sequence similarity search querying known AdhE sequences. The purple line and the grey shade represent the histograms for hit proteins showing >90% and 45–50% alignment coverages, respectively. The arrow indicates the peak of genes showing high alignment coverages but low identities with AdhE (namely Quasi-AdhE). b An experimentally determined monomer structure of AdhE (compact form; PDB ID: 6TQM) and a predicted monomer structure of Quasi-AdhE from Halomonas eurihalina by AlphaFold v2.3.2. Gene phylogenies of proteins possessing ALDH (c) or ADH (d) domain. We conducted multiple sequence alignment and phylogeny estimation for the union of 200 randomly sampled AdhEs and all 47 BdhEs (Quasi-AdhEs), as well as 10 proteins randomly selected for every KEGG Ortholog only with ALDH or ADH domains. The red, blue, and purple tips represent ALDH-only, ADH-only, and ALDH-ADH fusion proteins, respectively. The black and grey branches represent splits for which the Ultrafast Bootstrap values were ≧95% or not, respectively. Source data are provided as a Source Data file.
