Fig. 3: Monitoring of the scale-up enzymatic synthesis of Ψ as well as ΨMP and the convenient process for rapid purification of Ψ. | Nature Communications

Fig. 3: Monitoring of the scale-up enzymatic synthesis of Ψ as well as ΨMP and the convenient process for rapid purification of Ψ.

From: A combined pseudouridine biomanufacturing platform enabled by a streamlined designer pathway

Fig. 3

a Time course monitoring of Ψ synthesis (with a scale of 10 g UMP as substrate). b Time course monitoring of ΨMP synthesis (with a scale of 10 g UMP as substrate). c Time course monitoring of Ψ synthesis (with a scale of 10 g CMP as substrate). d Time course monitoring of ΨMP synthesis (with a scale of 10 g CMP as substrate). e The convenient process for rapid purification of Ψ produced in vitro. The crystallized Ψ product was further analyzed by HPLC. Elements were created in BioRender.

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