Fig. 4: Identification of tissue cells with cross-phenotypes and identified myeloid and NK cells in salivary glands. | Nature Communications

Fig. 4: Identification of tissue cells with cross-phenotypes and identified myeloid and NK cells in salivary glands.

From: Comparative single-cell and spatial profiling of anti-SSA-positive and anti-centromere-positive Sjögren’s disease reveals common and distinct immune activation and fibroblast-mediated inflammation

Fig. 4

a The UMAP plot displaying tissue cell types. b Heatmap illustrates expression levels of marker genes across different tissue cell types. c Expression levels of markers across tissue cell subsets. d Immunofluorescence images displaying the localization of specific markers in various tissue structures, including serous and mucous acini, ducts, myoepithelial cells, and stem cells. The last four figures are representative of cells with cross-phenotypes of different tissue cell subsets. e The UMAP plot displaying subsets of myeloid cells and NK cells in salivary glands. f Expression levels of markers across myeloid and NK cell subsets. g Differentially expressed gene (DEG) analysis in acinar (Serous and Mucous) and monocytes (classical (CL Mono) and non-classical monocytes (NC Mono)) across SjD subgroups compared to Sicca. Volcano plots show log₂ fold changes (logFC) (x-axis) and –log₁₀ adjusted p-values (y-axis) for DEGs between each SjD subgroup and Sicca controls in acinar (left three panels) and monocytes (right three panels). Upregulated genes (red) and downregulated genes (blue) are highlighted with selected key genes annotated. Genes not reaching significance are shown in grey. Source data are provided as a Source Data file.

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