Fig. 3: Zika infection induces a sex-dimorphic response in the mouse placenta.
From: Prenatal exposure to Zika virus shapes offspring neutrophil function in a sex-specific manner
At E8.5, pregnant mice were intraperitoneally injected with ZIKV (50 PFU/mouse), and placentas were collected for bulk RNA sequencing at E12.5. a PCA plot of female control placenta (n = 3), female ZIKV placenta (n = 5), male control placenta (n = 5), and male ZIKV placenta (n = 3), placentas were collected from 2-3 dams per group. b Heatmap showing top 20 most significant differentially expressed genes (DEGs) (adjusted p value < 0.05) in female and male responses. c Venn diagram of top biological processes unique to females and males’ response to ZIKV after high-specificity pruning. d Venn diagram of top pathways unique to females and males’ response to ZIKV after false discovery rate correction. e mRNA expression of IFN-β (n = 5 female, n = 6 male placentas from 3 control dams; n = 8 female, n = 6 male placentas from 3 ZIKV dams; p = 0.0077 male control vs. male ZIKV, p = 0.0022 female ZIKV vs. male ZIKV), Mx1 (n = 6 female, n = 9 male placentas from 3 control dams; n = 10 female, n = 9 male placentas from 3 ZIKV dams; p = 0.0342 male control vs. male ZIKV), Isg15 (n = 6 female, n = 7 male placentas from 3 control dams; n = 10 female, n = 7 male placentas from 3 ZIKV dams; p = 0.0455 male control vs. male ZIKV), and IL-1β (n = 6 female, n = 6 male placentas from 3 control dams; n = 9 female, n = 9 male placentas from 3 ZIKV dams; p = 0.033 female control vs. female ZIKV, p = 0.038 male control vs. male ZIKV, p = 0.0238 female control vs. male control, p = 0.0081 female ZIKV vs. male ZIKV) in E12.5 mouse placentas by qRT-PCR. Data are shown as the mean ± SD. Two-way ANOVA with Ĺ Ădák’s multiple comparisons was used. Source data are provided as a Source Data file.
