Fig. 5: Impact of maternal Zika infection on offspring neutrophil transcriptome.
From: Prenatal exposure to Zika virus shapes offspring neutrophil function in a sex-specific manner
a Experimental design. Bone marrow neutrophils were isolated from male and female offspring on PND35 from control and Zika-exposed groups for bulk RNA sequencing. Created in BioRender. Alvero, A. (2025) http://BioRender.com/nvwup3t. b Volcano plot of sex-dimorphic DEGs at baseline, DEGs in ZIKV-exposed male neutrophils, and DEGs in ZIKV-exposed female neutrophils. c Top 10 sex-dimorphic biological processes, molecular functions, and pathways comparing control female neutrophils and control male neutrophils. d Venn diagram of top DEGs unique to female and male offspring bone marrow neutrophils after in utero ZIKV exposure. Differential expression analysis was performed using DESeq2 with Benjamini–Hochberg correction for multiple comparisons. All tests were two-sided. The raw and processed RNA sequence data are available in NCBI’s Gene Expression Omnibus under the accession number GSE292966.
