Fig. 5: Characterization of the CNS border reporter (Aqp4-mRuby3; VE-cadherin-GFP) mouse.
From: In vivo imaging of the barrier properties of the glia limitans during health and neuroinflammation
Confocal images of immunofluorescence stainings of 20 µm thick brain cryosections collected from healthy Aqp4-mRuby3; VE-cadherin-GFP CNS border reporter mice. The AQP4-mRuby3 signal is seen in red and the GFP+ adherens junctions of the blood vessels and between leptomeningeal fibroblasts are seen in green. a, b Immunofluorescence staining for laminin (white) and DAPI staining for nuclei. Asterisks mark the lumen of leptomeningeal (a) or parenchymal blood vessels (b); n = 2. a XY MIP shows that the parenchymal basement membrane (white) colocalizes with the AQP4-mRuby3 signal from the superficial glia limitans (GL). VE-cadherin-GFP+ leptomeninges lie above of the GL. b First row, XY MIP of penetrating blood vessel. Second row, optical section of 2.2 μm. Third row, inset of ROI indicated by pink square. The VE-cadherin-GFP signal from endothelial cells (pink arrowheads) is surrounded by AQP4-mRuby3 signal from the perivascular GL (yellow arrowheads) and colocalizes with the parenchymal basement membrane.
