Fig. 5: Immunostaining of the different markers identified in the high resolution lung fibrosis mesothelial cell dataset.

a Immunostaining showing the AAV control specificity to the mesothelial cells. N = 6 biological replicates (C57BL/6J WT mice) and three independent experiments. Scale bar is 0.5 mm. b Immunostaining of α-sma of Bleomycin ctrl AAV group. Tile imaging was used to capture the lungs and performed measurements on fibrotic foci across at least 12 different regions of the affected lungs. N = 12 biological replicates (C57BL/6J WT mice) and three independent experiments. Data are presented as mean values ± SEM. Scale bar is 20 µm. Source data are provided as a Source Data file. c Immunostaining of α-sma in Ifi27l2a bleo day 14 group. N = 6 biological replicates (C57BL/6J WT mice) and three independent experiments. A two-sided independent T-test was used for the comparison of two groups. Data are presented as mean values ± SEM. Scale bar is 20 µm. Source data are provided as a Source Data file. d Immunostaining of Mmp8 in Dcn PBS day 14 group. N = 6 biological replicates (C57BL/6J WT mice) and three independent experiments. A two-sided independent T-test was used for the comparison of two groups. Data are presented as mean values ± SEM. Scale bar is 10 µm. Source data are provided as a Source Data file. e Experimental design of the collagen reporter AAV approach: AAV with col1 reporter was injected intrapleurally 4 day before the bleomycin instillation. At day 0 the bleomycin was administrated intratracheal and lungs were harvested 14 day after that. f Weight loss, survival curve and lung function of the different groups in the Col1 reporter experiment (from right to left). N = 6 biological replicates (C57BL/6J WT mice) and three independent experiments. A two-sided independent T-test was used for the comparison of two groups. Data are presented as mean values ± SEM. Source data are provided as a Source Data file. g Panel of trichrome staining and immunostaining of the Col1 reporter and the mesothelial cells. N = 6 biological replicates (C57BL/6J WT mice) and three independent experiments. Scale bar is 20 µm.Created in BioRender. Kadri, S. (2025) https://BioRender.com/p5gpax6.