Table 2 Comparison among Cas12-based one-pot detection methods
From: Tailoring Cas12a functionality with a user-friendly and versatile crRNA variant toolbox
Method | Cas12a Subtype | Amplifica-tion method | Time (min) | LoD (cp/uL) | Quantitative |
|---|---|---|---|---|---|
SONAR (This study) | LbCas12a | RPA | 20–30 | 0.6, 6, and 60 | Yes |
Photocontrolled assay40 | LbCas12a | RPA | 90 | 10 | No |
OAR-CRISPR56 | Cas12b | RPA | 8 | 60 | No |
AapCas12b mediated testing14 | AapCas12b | LAMP | 30 | 0.5 | No |
sPAMC15 | LbCas12a | RPA | 15 | 0.6 | No |
Glycerol-enhanced testing57 | LbCas12a | RPA | 60 | 0.6 | No |
AIOD-CRISPR58 | LbaCas12a | RPA | 20-40 | 3 | No |
CASMEN59 | LbaCas12a | RAA | 30 | 1 | No |
enAsCas12a and hybrid DNA-RNA guides11 | enAsCas12a | LAMP | 30 | 2–40 | No |
