Fig. 5: Transport across DpPorA and DpPorA DE pore in giant unilamellar vesicle system.

Schematic and representative images depicting the transport of Alexa Fluor 350 in a Control, b DpPorA, and c DpPorA DE. Graph showing the time-dependent analysis of normalized intensities of individual vesicles in the presence of d DpPorA (n = 8 vesicles each) and e DpPorA DE (n = 10 vesicles each). Statistical analysis of the percentage of permeabilization of hydrophilic fluorescent dyes in f DpPorA incorporated vesicles (n = 136, 64, and 72 individual vesicles for Alexa Fluor 350, Alexa Fluor 555, and ATTO 488 Dextran, respectively from N = 4 batches) and control vesicles (n = 123, 63, and 60 vesicles for Alexa Fluor 350, Alexa Fluor 555, and ATTO 488 Dextran, respectively from N = 4 batches). g DpPorA DE incorporated vesicles (n = 125, 62, and 63 vesicles for Alexa Fluor 350, Alexa Fluor 555, and ATTO 488 Dextran, respectively, from N = 4 batches) and control vesicles (n = 143,70 and 73 vesicles for Alexa Fluor 350, Alexa Fluor 555, and ATTO 488 Dextran, respectively, from N = 4 batches). The percentage of permeabilization values was denoted as mean ± S.D. The number of batches and the total number of vesicles are denoted as “N” and “n,” respectively. Buffer conditions: 100 mM KCl in 10 mM HEPES (pH: 7.4). Scale bar: 10 µm. Schematic figures of GUVs are created with BioRender.com.